Table 2. Primers used for the preliminary analysis of a subset of PNG samples.
| Gene | Primer | Sequence 5’-3’ | Product Size (nt) | ORF Region (nt) |
|---|---|---|---|---|
| tp0619 | Sense | 5’-TACAAGCTCCCACAATGCCA-3’ | ||
| Antisense | 5’-TTACCCAGACATTTTCTCCACATA-3’ | 6531 | 224–798 | |
| Sequencing | 5’-TACAAGCTCCCACAATGCCA-3’ | |||
| tp0136 | Sense | 5’-GAAGAGGGCGTTTTGTG-3’ | ||
| Antisense2 | 5’-CTCCCAGCTCAGCCGAATCTC-3’ | 16702 | 1–1485 | |
| Sequencing | 5’-GAAGAGGGCGTTTTGTG-3’ | |||
| 5’-CTCCCAGCTCAGCCGAATCTC-3’ | ||||
| 5’-CCATCCAGTCGGAAGTGC-3’ | ||||
| 5’-AACTACGTAGATTTTCTGCAC-3’ | ||||
| tp0326 | Sense | 5’-CTGACGGTGGGCTTTGAC-3’ | ||
| Antisense3 | 5’-GCATCTATGACGGCAAAGCG-3’ | 11693 | 1538–2559 | |
| Sequencing | 5’-AGCACGCCGTCTATTACCAG-3’ | |||
| 5’-AAGAGCATTCGTTTCGCTCC-3’ | ||||
| 5’-CTGACGGTGGGCTTTGAC-3’ |
1 Amplicon also contains tp0618-tp0619 intergenic region (58 nt) and 20 nt of tp0618
2 Amplicon also contains tp0135-tp0136 intergenic region (20 nt at 5’-end of tp0136) and 165 nt of tp0137
3 Amplicon also contains 148 nt of tp0327