Table 3. Primers used for the MLST scheme.
| Gene | Primer | Sequence 5’-3’ | Product Size (nt) | ORF Region (nt) |
|---|---|---|---|---|
| tp0548 | Sense1 | 5’-GGTCCCTATGATATCGTGTTCG-3’ | 300 | 130–212 |
| Antisense1 | 5’-CGTTTCGGTGTGTGAGTCAT-3’ | |||
| Sequencing | 5’-GTCATGGATCTGCGAGTGG-3’ | |||
| tp0136 | Sense | 5’-CCATCCAGTCGGAAGTGC-3’ | 223–675 | |
| Antisense2 | 5’-CATATCGAGAAACTGTTCGCC-3’ | 563 | ||
| Antisense3 | 5`-CGTGCAGGCAGAACTCATT-3’ | 464 | ||
| Sequencing | 5’-CCATCCAGTCGGAAGTGC-3’ | |||
| tp0326 | Sense | 5’-AAGAGCATTCGTTTCGCTCC-3’ | 441 | 2031–2342 |
| Antisense | 5’-CCGGACCGTAGCTCATTTTG-3’ | |||
| Sequencing | 5’-GACACCAAGGCCGAGTTCTA-3’ |
1 Published tp0548 primers [11]
2 Antisense primer for tp0136 subtypes A-F
3 Antisense primer for tp0136 subtype G