Fig 2. Sequential infection with MCMV followed by Ad-LacZ.

(A) C57BL/6 mice were first infected with MCMV, then >50 days later were infected with Ad-lacZ. The size of the pre-existing MCMV specific M38 effector memory response after Ad-lacZ infection was measured in the blood and liver by ex-vivo tetramer staining. The percentage of pre-existing M38 response in the blood and (B) the absolute numbers in the liver after immunization with Ad-lacZ vector was determined at the indicated time points. (C) The kinetics and magnitude of the new inflating response (D8V) in groups of mice with latent MCMV was also measured by ex vivo tetramer staining compared against uninfected mice. The percentage of D8V tetramer+ cells in the blood and (D) the absolute numbers in the liver was measured at the indicated time points. The figures show the mean from 3–8 mice per time point obtained from 2 independent experiments. p values were measured by Mann-Whitney tests. *p<0.05.