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. Author manuscript; available in PMC: 2018 Nov 8.
Published in final edited form as: J Am Chem Soc. 2017 Oct 25;139(44):15681–15690. doi: 10.1021/jacs.7b06416

Figure 5.

Figure 5

LC-HCD/UVPD analysis of two synthetic standards (PC 16:0/18:1(n-9) and .PC 18:1(n-9)/16:0) present within an artificial mixture of standards mixed in a 1:1 ratio. (A) The base peak chromatogram (BPC) for LC-HCD/UVPD of m/z 782. (B) The extracted ion chromatogram (XIC) for HCD/UVPD product ions of m/z 345, indicative of PC 18:1(n-9)/16:0, is shown in blue, while the XIC of m/z 319, indicative of PC 16:0/18:1(n-9) is shown in green. (C) The HCD/UVPD spectrum obtained by integrating across the chromatographic peak between 19.7 and 19.9 min. (D) The HCD/UVPD spectrum obtained by integrating across the chromatographic peak between 20.2 – 20.4 min.