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. Author manuscript; available in PMC: 2018 Jan 9.
Published in final edited form as: Neuron. 2017 Aug 17;95(5):1074–1088.e7. doi: 10.1016/j.neuron.2017.07.038

Figure 4. dVGLUT Localizes to Subpopulations of DA Nerve Terminals.

Figure 4

Fluorescent tags were co-expressed by dVGLUT-LexA (mCherry) and TH-GAL4 (mCD8::GFP) enhancer-driven expression drivers in WT back-ground.

(A) Representative image from a projected z series highlights co-localization of dVGLUT- and TH-promoter driven fluorescent tags in the DA terminal-rich MB-MV1 region (boxed in white; 20 µm scale bar; false color).

(B) Single-channel images zoomed in on MV-MB1 show TH-GAL4-driven mCD8::GFP (top left; green), VGLUT-LexA-driven mCherry (top right; red), and nc82 (labeling synaptic active zone marker Bruchpilot; bottom left; blue). Merged image (bottom right) shows co-localization of these fluorescent tags and nc82 labeling in DA terminal active zones. Fly strain: VGLUT-LexA/LexAOP-6x mCherry;TH-GAL4/UAS-mCD8::GFP. Data are representative of n > 3 experiments.

See also Figures S2 and S3.