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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: FEBS J. 2017 Nov 23;285(1):101–114. doi: 10.1111/febs.14321

Figure 1. TGFBIp and POSTN expression in the cornea.

Figure 1

(A) In situ hybridization analysis of TGFBI and POSTN mRNA in wildtype mouse corneas. A strong signal (indicated via silver grains) for TGFBI mRNA is restricted to the corneal epithelium, whereas no apparent signal is observed for POSTN mRNA (scale bar = 20μm). (B) Real-Time PCR supports the presence of TGFBI and the lack of POSTN mRNA in isolated corneal tissues (n = 3). Error bars represents the SD. (C) Immunohistochemically staining using a POSTN antibody verifies the absence of POSTN in the central cornea and reveals that POSTN protein is localized at the corneal periphery. Moreover, the extent of POSTN deposition in wildtype and TGFBI−/− mice corneas is unchanged, whereas no POSTN was detected in POSTN−/− corneas (scale bars = 100μm).