Skip to main content
. Author manuscript; available in PMC: 2019 Jan 8.
Published in final edited form as: Dev Cell. 2017 Dec 14;44(1):13–28.e3. doi: 10.1016/j.devcel.2017.11.011

Figure 1. Expression of Nf2 in differentially growing optic neuroepithelial compartments.

Figure 1

(A) Sections of mouse embryonic heads and post-natal eyes (C57BL/6J) were immunostained to identify the cells possess mitotic cell cycle marker pH3 and DNA-labeled with BrdU. Nuclei are visualized by DAPI staining. NR, neural retina; pNR, presumptive neural retina; RPE, retinal pigmented epithelium; pRPE, presumptive retinal pigmented epithelium; CM, ciliary margin; ICM, inner ciliary margin; OCM, outer ciliary margin; CB, ciliary body. (B) Quantification of BrdU-positive cell population in the each optic compartment at indicated ages. Error bars in the graphs represent mean ± SEM (n = 6 from 4 independent litters). (C) Expression of Nf2 in the mouse eyes at the indicated ages was examined by immunostaining. Arrows point the Nf2 immunostaining signals. dOV, dorsal optic vesicle; vOV, ventral optic vesicle; LV, lens vesicle; IS, inner segment; OS, outer segment; dOS, dorsal optic stalk; vOS, ventral optic stalk; PCE, pigmented ciliary epithelium; NPCE, non-pigmented ciliary epithelium. (D) Nf2 distribution in the entire ICM (marked by Cdo, left), proximal ICM (marked by Msx1, center), and distal ICM (marked by Aqp1, right) was determined by co-immunodetection of Nf2 and representative ICM markers. Arrowheads indicate the proximal margins of Nf2 staining signals.