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. Author manuscript; available in PMC: 2019 Jan 8.
Published in final edited form as: Dev Cell. 2017 Dec 14;44(1):13–28.e3. doi: 10.1016/j.devcel.2017.11.011

Figure 4. Autonomous expansion of the CM population in the absence of Nf2.

Figure 4

(A and G) Nf2 immunoreactivity and R26lacZ Cre recombinase activity reporter expression in the eyes of E14.5 Nf2f/+;Chx10-Cre;R26R and Nf2f/f;Chx10-Cre;R26R littermates (A) and P0 Nf2f/+;MART1-Cre;R26R and Nf2f/f;MART1-Cre;R26R littermates (G) were examined. Nf2 immunoreactivity is eliminated in R26R-positive ICM cells (arrowheads in A) of E14.5 Nf2f/f;Chx10-Cre eyes, but it remains in R26R-negative OCM cells (arrow in A). Nf2 immunoreactivity is eliminated in R26R-positive OCM cells (arrowheads in G) of P0 Nf2f/f;MART1-Cre eyes. (B and H) H&E staining images of eye sections of P7 Nf2f/+;Chx10-Cre and Nf2f/f;Chx10-Cre littermates (B) and P7 Nf2f/+;MART1-Cre and Nf2f/f;MART1-Cre littermates (H). (C and I) Sections of P0 Nf2f/+;Chx10-Cre;R26R and Nf2f/f;Chx10-Cre;R26R mouse eyes (C) and P0 Nf2f/+;MART1-Cre;R26R and Nf2f/f;MART1-Cre;R26R mouse eyes (I) are stained with antibodies against Pax6, Vsx2, and Otx2. CM areas are outlined by solid lines, and the borders between ICM and OCM are marked by dot-lines. (D and J) Quantification of Pax6-positive (left), Vsx2-positive (center), and Otx2-positive (right) cells in the Nf2f/+;Chx10-Cre;R26R and Nf2f/f;Chx10-Cre;R26R mouse eyes (D) and Nf2f/+;MART1-Cre;R26R and Nf2f/f;MART1-Cre;R26R mouse eyes (J) at indicated ages (n = 6 from 3 independent litters). (E and K) Nf2f/+;Chx10-Cre and Nf2f/f;Chx10-Cre (E) and Nf2f/+;MART1-Cre and Nf2f/f;MART1-Cre (K) mouse eyes from P0 and P7 mice were analyzed to detect proliferating CM cells, which are positive to BrdU and Pax6. (F and L) Quantification of BrdU-positive cells in the whole CM (left), ICM (middle), and OCM (right) of Nf2f/+;Chx10-Cre and Nf2f/f;Chx10-Cre (F) and Nf2f/+;MART1-Cre and Nf2f/f;MART1-Cre (L) mouse eyes (n = 6 from 3 independent litters).