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. Author manuscript; available in PMC: 2019 Jan 8.
Published in final edited form as: Dev Cell. 2017 Dec 14;44(1):13–28.e3. doi: 10.1016/j.devcel.2017.11.011

Figure 6. Yap/Taz haplodeficiency rescues CM hyperplasia caused by Nf2 deficiency.

Figure 6

(A) Sections of mouse eyes at indicated ages were stained with an antibody against Yap/Taz. Single focal plane images are provided to identify subcellular distribution of Yap/Taz. Note that RPE cells show very limited amount of Yap/Taz expression in the nucleus (white arrows), whereas CM cells exhibits expression in the nucleus as well as cytoplasm (yellow arrows). (B) P0 Lats1f/+;Lats2f/+, Lats1f/f;Lats2f/+;MART1-Cre and Lats1f/+;Lats2f/f;MART1-Cre littermate mouse eye sections were stained with antibodies recognizing corresponding markers. Arrows point Yap/Taz in the OCM. (C and D) The numbers of CM cells expressing corresponding markers in the mouse eyes were quantified (n = 4 from 2 independent litters). (E and H) Sections of P0 mouse eyes with indicated genotypes were stained with H&E and antibodies against corresponding markers. The levels of Yap/Taz are higher in the ICM (E, arrowheads in third row) than the OCM of P0 wild-type mouse eyes. Yap/Taz are elevated and strongly accumulated in the nuclei of the OCM and RPE cells in P0 Nf2f/f;TRP1-Cre mice (E, arrows in third row). Yap/Taz were strongly accumulated in the nuclei of the OCM of P0 Nf2f/f;MART1-Cre mice (H, arrows in third row). (F and I) Pax6-positive total CM cells, Otx2-positive OCM cells, and Vsx2-positive ICM cells in P0 mouse eyes with indicated genotypes are quantified and shown in a graph (n = 5 from 2 independent litters). (G and J) Quantification of BrdU;Pax6-positive cells in total CM cells, OCM, and ICM area of P0 mouse eyes with indicated genotypes (n = 5 from 2 independent litters). n.d., not detectable.