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. Author manuscript; available in PMC: 2019 Jan 15.
Published in final edited form as: J Immunol. 2017 Dec 1;200(2):758–767. doi: 10.4049/jimmunol.1700713

Fig. 4.

Fig. 4

Impact of toll-like receptor 2 (TLR2) receptor stimulation on CD44 gene expression and CD44 levels in THP-1 macrophages and the role of hyaluronan (HA). TLR2 receptor stimulation was performed using Pam3CSK4 (Pam; 5ng/mL) for 24 hours. Data represents the mean ± standard deviation of 4 independent experiments. *p<0.001; **p<0.01.

A) HA (100, 500 and 1,000 μg/mL) treatments reduced TLR2 induced CD44 gene expression in THP-1 macrophages.

B) A representative flow cytometry histogram demonstrating increased CD44 protein levels in TLR2 stimulated THP-1 macrophages. HA (500 and 1,000 μg/mL) treatments reduced CD44 levels in TLR2 stimulated THP-1 macrophages.

C) Semi-quantitative analysis of mean fluorescence intensities of CD44 receptor following TLR2 stimulation in the absence or presence of HA (100, 500 or 1,000 μg/mL). TLR2 stimulation increased CD44 protein in THP-1 macrophages and HA (500 and 1,000 μg/mL) treatments reduced CD44 protein.