Figure 4. Ripk1^{DC KO} mice develop inflammation that is partially TNF-dependent.

(A) BMDCs from Ripk1^{DC KO} Tnfr1^−/− but not Ripk1^{DC KO} Ifngr^−/− mice are protected from necroptosis in vitro. BMDCs were treated with SMAC mimetics and zVAD-fmk. Death measured at 20 h by Cell Titre Glo. N=3. (B) Spleen and lymph node weights and lymph node cellularity of mice of the indicated genotypes. (C) Neutrophils and inflammatory monocyte numbers in the spleens of mice with the indicated genotypes. (D) H&E and Masson’s trichrome staining of spleen sections from Ripk1^{DC KO} Tnfr1^−/−, Ripk1^{DC KO} Ifngr^−/− and control mice. (E) Numbers of neutrophils, inflammatory monocytes and B cells in the lymph nodes of mice of the indicated genotypes. (F) Proportion of mice serum positive for ANAs at 6 months (n=3–6 mice per genotype). CD11cCre and Ripk1^{DC KO} mouse phenotyping data used in Fig. 4B, C, E, F were shown in Fig. 2. Error bars, means ± SEM. Scale bars represent 100μm. Unpaired two-tailed Student’s t test (A–C, E). * p<0.05; **p<0.01.