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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Microcirculation. 2018 Jan;25(1):10.1111/micc.12431. doi: 10.1111/micc.12431

Figure 2. The protein expression of KV1 channel-forming α-subunits in human coronary arteries.

Figure 2

Western blot analysis of KV1 α-subunits was performed using the membrane fraction of non-CAD HCAs. Consistent with high mRNA expression, KVα1.5 protein was detected in HCAs. KVα1.3, 1.4 and 1.6 were also detected in these samples. Arrow indicates mature forms of KV1 proteins, with the approximate molecular mass values as follows: 75 kDa doublet (1.1), 75 kDa (1.2), 75 kDa doublet (1.3), 110 kDa doublet (1.4), 75 kDa (1.5), and 60 kDa (1.6). Proteins prepared from human brain tissue and HEK-293 with (+) or without (-) human KV1.5 overexpression were included as positive controls.