Table 2.
Nucleotide sequence of primers used for PCR
| For end-point PCR | ||
|---|---|---|
|
| ||
| Transcript | Forward and Reverse primers | Amplicon size, bp |
| KV α1.1 | 5′-AGT CGC ACT TCT CCA GTA TCC-3′ 5′-TCG GTC AGT AGC TTG CTC TTA T-3′ |
424 |
| KV α1.2 | 5′-TCA AGT TGT CCA GAC ACT CCA A-3′ 5′-TGT GTT AGC CAA GGT ACA GTT G-3′ |
548 |
| KV α1.3 | 5′-CTT CAG GTT TCA GCA GCA TCC-3′ 5′-GCA GGT GGC AGT GGA ATT G-3′ |
395 |
| KV α1.4 | 5′-GAG GCG GAT GAA CCT ACT ACC-3′ 5′-CCC TTT CCC TGA CAC TTC TCC-3′ |
401 |
| KV α1.5 | 5′-CCG TCT ACT TCG CAG AGG CT-3′ 5′-CCA GGC AGA GGG CAT AAA GG-3′ |
465 |
| KV α1.6 | 5′-AGA GGC TGA CGA TGA CGA TTC-3′ 5′-CCG ATG TGG TGT AGG AAG GTA G-3′ |
364 |
| KV α2.1 | 5′-GGC TTG CTC ATC CTC TTC CTT-3′ 5′-TCT CCT GTC TCT TCT GCT CCT T-3′ |
289 |
| KV α9.3 | 5′-GGC TTC TGC TTC TCT TCC TCT-3′ 5′-ACT GGT CCA CAT CAA TGT CCT T-3′ |
297 |
| KV β1.1 | 5′-CAG CAG CCT TAG TCC CTC AG-3′ 5′-GCC GTT CAG CAA CCT CAT CT-3′ |
239 |
| KV β1.2 | 5′-GTG GAG CAG CCG AAC AGA A-3 5′-GCC GTT CAG CAA CCT CAT CT-3′ |
192 |
| KV β1.3 | 5′-ACC TCT CGT GAC TGC CTG T-3′ 5′-GCC GTT CAG CAA CCT CAT CT-3′ |
378 |
| BKCaα | 5′-ATG CGG AAC TCA CCC AAC A-3′ 5′-TCG CCA AAG ATG CAG ACC AC-3′ |
224 |
| β-actin | 5′-GCT CGT CGT CGA CAA CGG CTC-3′ 5′-CAA ACA TGA TCT GGG TCA TCT TCT C-3′ |
353 |
|
| ||
| For real-time PCR (qPCR) | ||
|
| ||
| Transcript | Forward and Reverse | Amplicon size, bp |
|
| ||
| KV α1.5 | 5′-CGTCATCGTCTCCAACTTCAAC-3′ 5′-TTCCGCTGGACTCCTCTGT-3′ |
126 |
| ATP synthase O subunit | 5′-TCTCCTTTAGAAGAAGCCACACT-3′ 5′-GCACAATCATTCCACCCAAGAT-3′ |
124 |
| β-actin | 5′-GCACTCTTCCAGCCTTCCTT-3′ 5′-TGTGTTGGCGTACAGGTCTT-3′ |
114 |