Figure 4. SERCA2a 971-990 induces CD4 T cell response.

Groups of mice were immunized with or without SERCA2a 971-990. At termination on day 21, LNCs and splenocytes were prepared, and CD4 T cells were enriched from immunized animals by magnetic separation. Cells were stimulated with SERCA2a 971-990 or RNase 43-56 (control) for two days and after pulsing with ³[H]-thymidine for 16 hours, proliferative responses were measured as cpm. Data from three individual experiments each involving 3 mice are shown for LNCs and splenocytes, and for CD4 T cells, data obtained from one of the two experiments involving 8 mice are shown. Comparisons were made between SERCA2a 971-990 and medium control and RNase 43-56. **p< 0.01.