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. Author manuscript; available in PMC: 2019 Jan 15.
Published in final edited form as: J Immunol. 2017 Dec 8;200(2):704–714. doi: 10.4049/jimmunol.1601559

Figure 2. TIRAP undergoes poly-ubiquitination and multiple mono-ubiquitinations in the presence of CLIP170.

Figure 2

HEK293T cells were co-transfected with FLAG-TIRAP, CLIP170 and various Lysine to Arginine mutants of HA-Ubiquitin followed by immunoprecipitation of FLAG-TIRAP and immunoblotting. CLIP170 mediated conjugation of all the mutant versions of Ubiquitin including UBI7KR where all the Lysine residues are mutated to Arginine. The vertical white lines indicate where the figure panels were joined.