Fluorescence analysis of Emodin-DNA interactions.
(a) Intrinsic fluorescence emission spectra of DNA: EB (75:6.3 μM) excited at 350 nm in the presence of various concentrations (A–K represent 0, 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 μM respectively) of Emodin and L (red dotted line) represents emission spectra of 50 μM emodin alone excited at 350 nm, (b) Stern-Volmer plot for quenching of DNA: EB complex by emodin, (c) Modified Stern-Volmer plot for quenching of DNA: EB complex by Emodin.