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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Mol Microbiol. 2017 Dec 8;107(2):164–179. doi: 10.1111/mmi.13868

Figure 5. NepR interaction with unphosphorylated PhyR affects NepR structure.

Figure 5

1H-15N HSQC spectra of uniformly 15N-labeled C. crescentus NepR; CLEANX-PM spectra for all samples are shown in black. (A) NepR alone (blue); (B) NepR in the presence of excess unlabelled, unphosphorylated C. crescentus PhyR (green, arrows indicate new, shifting, or lost peaks); (C) NepR in a complex with unlabeled PhyR~P, denoted PhyR~P:15N-NepR (red).