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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Addict Biol. 2017 Jul 9;23(2):810–823. doi: 10.1111/adb.12530

Figure 1.

Figure 1

(A) Experiment timeline. Animals in Group 1 were exposed to adolescent intermittent ethanol (AIE; 5 g/kg ethanol [EtOH], 25% v/v in water, i.g., 2-day-on, 2-day-off, postnatal day [P]25-P54). Control (CTRL) animals in Group 2 received an equivalent volume of water during the same exposure regimen. In adulthood, each animal underwent two scanning days. (B) Procedure on each scan day. Three scans were acquired in immediate succession: 1) 10-minute baseline resting-state scan [PreChall], 2) 25-minute challenge scan [Chall], and 3) 10-minute post-challenge scan [PostChall]. During the Chall scan, animals were injected i.p. with either 2 g/kg EtOH (20% v/v) or an equivalent volume of saline (SAL) 6 minutes after scan start. Each animal served as its own saline control for acute EtOH challenge, and the order of injection was counterbalanced.