Figure 1.

Translational inhibition of inducible nitric oxide synthase (iNOS) is reversely correlated with miR-146a expression. RENCA, CT26, and TRAMP-C2 tumor cells lines (10⁶ cells), and RAW 264.7 macrophage-like cells (10⁶ cells) were incubated with or without the combined stimulation of IFNγ (100 U/ml) and LPS (1 µg/ml) for 24 h. (A) A representative western blot analysis for iNOS expression and (B) a representative histogram depicting EMMPRIN expression in RENCA cells (light gray, isotype control; blue, no stimulation; hatched yellow, with the combined stimulation). (C) Accumulation of nitrites, the stable product of nitric oxide (NO), reflecting inducible nitric oxide synthase (iNOS) activity; (D) densitometric analysis of western blots for iNOS protein expression; (E) iNOS mRNA accumulation; (F) accumulation of miR-146a-5p expression; (G) mean fluorescence of EMMPRIN protein expression; (H) accumulation of EMMPRIN mRNA (n = 5–6 in each group).