Figure 7.

Neutralization of miR-146a-5p, together with pro-inflammatory stimulated macrophages, inhibits tumor growth. (A) RENCA cells (2 × 10⁶ cells) were injected to the flank of BALB/c mice. After tumors became palpable, mice were i.v. injected every 7 days (black arrows), with either anti-miR-146a-5p or its negative control anti-miR-NC (0.025 mg/g BW each), alone or together with injections of RAW 264.7 (10⁶ cells) that were stimulated in vitro with IFNγ (100 U/ml) and LPS (1 µg/ml) for 24 h prior to injection. *p < 0.05, ***p < 0.001 relative to the control group, $, p < 0.05 relative to the anti-miR-146a-5p group. Representative images of tissue sections immunohistochemically stained for (B) EMMPRIN protein expression and (C) its evaluation by the h-score, and (D) inducible nitric oxide synthase (iNOS) protein expression and (E) its evaluation by the H-score (n = 6 in the miR-NC+stimulated RAW 264.7 group, and n = 5 in each of the other groups, in two biological replicates).