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. 2018 Jan 5;8:1901. doi: 10.3389/fimmu.2017.01901

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Copyright © 2018 Liu, Gallo, Khan, Renukaradhya and Brutkiewicz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparable numbers of iNKT cells in wildtype (WT) and Nf1^+/− mice. (A) Thymocytes, splenocytes, and liver mononuclear cells from Nf1^+/− mice and WT littermates were stained with α-GalCer-loaded CD1d tetramers and a TCR-β-specific antibody for the identification of iNKT cells, identified in the upper right quadrant. (B) Percentages (upper) and total numbers (lower) of iNKT cells are summarized for the thymus, spleen and liver. Pooled data from three independent experiments are shown. Each dot represents an individual mouse. The data are plotted as mean ± SEM.