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. 2018 Jan 5;8:1109. doi: 10.3389/fphys.2017.01109

Figure 1.

Figure 1

Experimental procedures for each compound tested in each tissue. Baseline APs were collected and assessed for 31 min in vehicle control solution (Tyrode with 0.1% DMSO) at stimulation frequencies of 1 Hz for 25 min, 2 Hz for 3 min and then 1 Hz for 3 min. Following baseline, 4 concentrations of a test compound were applied sequentially and cumulatively. Each concentration was applied for 31 min with the same stimulation paradigm as in control condition.