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Uniform illumination of high optical density samples. The intensity of the Hε protons of 5 mM N-Acetyl-L-Tyrosine (AcTyr) are shown for increasing concentrations of photosensitizer (FMN). Above 0.5 mM FMN the resulting solutions are of high optical densities, but the small active volume (1 μL) allows a uniform light penetration. Increasing FMN concentrations (from 0.2 to 2.0 mM, see legend) results in higher hyperpolarization effects on the Hε protons of AcTyr
