Figure 3.
Immunofluorescent observation of the expression of CD11b, F4/80, and GATA6 in peritoneal macrophages after CPT-11 treatment. (A) C57BL/6 mice (six mice per group) were injected intraperitoneally with CPT-11 at a dose of 200 mg/kg body weight. Peritoneal exudate cells were collected and planted in dishes (5 × 105/dish) ex vivo while non-adherent cells were discarded 2 h later. Then the attached cells (macrophages) were stained with antibodies against CD11b (green), F4/80 (magenta), and GATA6 (red). The cells were observed under a Zeiss fluorescent microscope, with the nuclei (blue) being revealed by Hoechst 33342, and the fluorescence images were captured and analyzed by the Zeiss ZEN software. Scale bar: 10 mm. (B) The ratios of GATA6+ cells were calculated using the Zeiss ZEN software. n = 6; *P < 0.05; ***P < 0.001; ns, not significant.