Figure 6.

Immunofluorescence observation of the expression of GATA6 in the peritoneal macrophages after CPT-11 treatment and syngeneic adoptive transfer with peritoneal exudate cells (PECs) and bone marrow cells (BMCs). C57BL/6 mice were intraperitoneally injected with CPT-11 (200 mg/kg body weight, in phosphate-buffered saline containing 2% Tween-80) or vehicle. Seven days later, the CPT-11-treated mice were transferred with syngeneic PECs (1 × 10⁶/mouse) or BMCs (1 × 10⁷/mouse). The mice were bred for additional 21 days, and then their PECs were collected. The expression of CD11b (green), F4/80 (magenta), and GATA6 (red) was observed by immunofluorescence microscopy with the nuclei (blue) being revealed by Hoechst 33342 staining. (A) A representative set of immunofluorescence images. Scale bar: 10 mm. (B) Statistical analysis of the percentages of CD11b⁺GATA6⁺ macrophages. n = 6; ***P < 0.001; ns, not significant.