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. 2018 Jan 5;8:1919. doi: 10.3389/fimmu.2017.01919

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Copyright © 2018 Bai, Li, Zhang, Xu, Zeng, Hu, Hong, He and Ouyang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of the B cell populations by flow cytometry after the syngeneic adoptive transfer with peritoneal exudate cells and bone marrow cells. C57BL/6 mice were treated as described in Figure 5. (A) The B cell populations (including B1 and B2 subpopulations) in the peritoneal cavity were analyzed by flow cytometry. B cell populations were gated as CD19⁺ cells, among which B1 cells were CD19⁺CD23⁻ ones while B2 cells were CD19⁺CD23⁺ ones. (B, C) and (D) Statistical analysis of the B (B), B1 (C), and B2 (D) cell counts in each group. n = 6; *P < 0.05; ***P < 0.001; ns, not significant.