FIGURE 4.

Complementation of a salinity-sensitive yeast mutant by OsHAK1. (A) Yeast cells harboring an empty vector (pYES2) are shown in the upper row, and those harboring OsHAK1 in the lower row. The (B) [K⁺] and (C) [Na⁺] of yeast cells grown either in the absence (control) or presence of 50 mM NaCl. Data represent the mean ± SE (n = 3). Significant differences (P < 0.05) between the performance of cells harboring the empty vector and those harboring the OsHAK1 transgene are indicated by an asterisk. ns, non-significant difference; DW, dry weight.