Table 4.
The primer sequences for amplifying variable regions 3 and 4 of the bacterial 16S ribosomal DNA sequences.
| Adaptor sequence | Spacer | Annealing sequence | |
|---|---|---|---|
| Forward primer | 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG | CCTACGGGNGGCWGCAG | |
| 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG | A | CCTACGGGNGGCWGCAG | |
| 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG | TD | CCTACGGGNGGCWGCAG | |
| 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG | GDR | CCTACGGGNGGCWGCAG | |
| Reverse primer | 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG | GACTACHVGGGTATCTAATCC | |
| 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG | T | GACTACHVGGGTATCTAATCC | |
| 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG | AC | GACTACHVGGGTATCTAATCC | |
| 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG | GTT | GACTACHVGGGTATCTAATCC |