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. 2018 Jan 9;8:184. doi: 10.1038/s41598-017-18000-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MRI does not show any sign of radionecrosis or radio-induced edema. Representative T1-weighted post-contrast (A) and T2-weighted (B) MRI performed 3 months after irradiation of the left dorsal hippocampus are shown. Blue lines indicate the irradiation zone in the left dorsal hippocampus. Note the absence of any sign of brain damage induced by the microbeams (peak dose: 600 Gy). Quantification of left and right dorsal hippocampal volumes in control unirradiated rats and in irradiated rats. Values were calculated directly from the DICOM images and are the means ± SD (n = 2–4). Statistical analysis was performed by Two-Way ANOVA (p = 0.992, irradiation x brain area) (C).