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. 2017 Dec 1;54(1):32–40. doi: 10.1007/s11626-017-0213-7

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Figure 2. — ×4 phase contrast images of SVF grown on Matrigel at 18 h (A, B); 36 h (C, D); 60 h (E, F); and 112 h (G, H). Images B, D, F, and H are ×3 optical zooms of boxed sections of images A, C, E, and G, respectively. White arrows demonstrate initial cell clustering occurring at 18 h. Tip cell formation occurs at 36 h as marked by the red arrow. Stalk cell formation and elongation occurs at 60 h as marked by the blue arrow. Branching (green arrow) and inosculation (yellow arrow) can be seen at 118 h in vitro.