Fig. 1.

Histone crotonylation is found in the intestine. a Western blot analysis of whole cell extracts from several mouse tissues using indicated antibodies shows that histone crotonylation is particularly abundant in the brain and colon; the analysis of tissues from two mice is shown. b Relative abundance of H3K18cr in the intestinal epithelium cell fractions, n = 3, error bars are standard deviation. c, d Immunofluorescence microscopy with anti-pan crotonyl antibody (green, left panels) and DAPI counterstaining (cyan, right panels) of a mouse colon (c) and small intestinal (d) tissue sections, scale bars 40 μm