Fig. 4.

Butyrate and class I HDAC inhibition promote histone crotonylation. a Western blot analysis with indicated antibodies of whole cell extracts of small intestinal organoids treated for 48 h with indicated amounts of SCFAs. Representative western blot of two repeat experiments. b HCT116 cells were treated with MS275 or DMSO (vehicle) for 18 h, whole cell extracts collected, and analyzed by western blot using indicated antibodies; anti-Kcr: anti-crotonyl-lysine antibody, NT: not treated. c Increase in histone H3K18cr over promoters of indicated genes and repetitive, heterochromatic sites (alpha-satellite sequences, NBL2) upon MS275 treatment of HCT116 cells for 18 h. Summary of ChIP-qPCR data of three repeat experiments, error bars are SEM