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. 2018 Jan 9;8:122. doi: 10.1038/s41598-017-18522-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Agar gel model insertion with and without the guide. The microprobes were inserted using a stereotactic arm with a micropositioner. The microprobes were lowered to just above the surface of the gel, and inserted at a speed of ~1 mm/s. (A) Example of a failed insertion attempt without the guide in-place. Note the dummy microprobe buckling as it makes contact with the surface of the 0.6% agar model. (B) Example of a successful insertion with guide in place. (C) Successful rate of insertion with and without guides. (D) Rate of trials resulting in any buckling regardless of insertion status.