Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 9;8:204. doi: 10.1038/s41598-017-18509-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Long-term transgene expression efficiency in mMSCs after lentiviral vector transduction. (a) The mMSCs were transduced separately with pHBLV-CMVIE-CXCR7-ZsGreen-T2A-Puromycin (MSC-OE-CXCR7), pHBLV-CMVIE-ZsGreen-T2A-Puromycin (MSC-OENC-CXCR7), pHBLV-U6-ShRNA-ZsGreen-Puromycin (MSC-Sh-CXCR7) and pHBLV-U6-ZsGreen-Puromycin (MSC-ShNC-CXCR7) lentiviral vectors were cultured for 20 passages and observed with light microscopy (top) and fluorescence microscopy with green fluorescent protein (middle), 100× and 250×; the percentage of ZsGreen-positive cells were analysed by flow cytometry (bottom) at passage 20 after transduction. (b) Quantitative real-time PCR analysis shows CXCR7 mRNA expression in mMSCs after pHBLV-CMVIE-CXCR7-ZsGreen-T2A-Puromycin and pHBLV-U6-ShRNA-ZsGreen-Puromycin transduction. (n = 4; *p < 0.05 vs. MSC-OENC-CXCR7, ^# p < 0.05 vs. MSC-Sh-CXCR7). (c,d) FCM analysis shows CXCR7 overexpression in mMSCs after transduction. (n = 3; *p < 0.05 vs. MSC-OENC-CXCR7). (e,f) FCM analysis shows CXCR7 suppression in mMSCs after transduction. (n = 3; ^# p < 0.05 vs. MSC-ShNC-CXCR7).