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. 2018 Jan 9;8:217. doi: 10.1038/s41598-017-18641-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Combination of SEP and αPD-L1 on T cell subsets from tumor in B16-F10-bearing mice. The mouse tumors were harvested at the end of the experiment (day 16). The cell counts for CD3⁺, CD4⁺ and CD8⁺ T cells from mouse tumor were determined by flow cytometry. The increase in the percentage of either CD3⁺, CD4⁺ or CD8⁺ T cells was observed in three treatment groups (each treatment P < 0.05 for each T cell, respectively, n = 6). And, combination of SEP and αPD-L1 had significantly additive increase of either CD3⁺, CD4⁺ or CD8⁺ T cells compared with SEP (each T cell P < 0.05, respectively, n = 6) or αPD-L1 (each T cell P < 0.05, respectively, n = 6). The results were presented as mean ± SEM. ^* P < 0.05 compared with control group. ^# P < 0.05, compared with SEP group. ^& P < 0.05, compared with αPD-L1 group.