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. 2018 Jan 9;8:144. doi: 10.1038/s41598-017-18644-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of ROS inhibition using Mito-TEMPO on the vital apoptotic markers at translational and transcriptional level. (a) Cellular levels of protein markers JNK, p-JNK, Bax, Bcl2, Cyt C, caspase-3, caspase-9, and β- actin in A549 cells incubated with Mito-TEMPO (10 μM). Densitometry analysis of the respective proteins was evaluated by Image J software, and results were normalized with β-actin with respect to controls. (b) Quantitative RT-PCR (qRT-PCR) analysis of bax, bcl2, cyt c, caspase-9, and β-actin after treatment with CANE in conjunction with Mito-TEMPO. Mito-TEMPO controls expression of apoptotic genes at transcription level represented in fold change compared with control. Each value in the graphs represents as the mean ± SD of three independent experiments. Values with different superscripts differ significantly from each other (p < 0.05).