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. 2018 Jan 9;8:160. doi: 10.1038/s41598-017-18188-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogeny of the Mycobacterium tuberculosis complex isolate consensus sequences (n = 18) annotated with loss of function mutations in MTase genes. A maximum likelihood phylogenetic tree, with the % of methylated motifs and potential loss of function mutations in MTase genes annotated. Allele frequencies of putative methylation related mutations across a global collection of M. tuberculosis isolates; *EAI6 stains, **lineages 4.3 to 4.9, - indicates absence.