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. 2018 Jan 9;8:151. doi: 10.1038/s41598-017-18532-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Performance of microscopy and PCR for the Trypanosoma cruzi detection in insect vectors: sensitivity and specificity as estimated through site-occupancy modelling. EPRS, entomological-parasitological routine surveillance; UnB, University of Brasília; cPCR rDNA-24Sα, conventional PCR on the 24Sα subunit of the nuclear ribosomal DNA; qPCR nDNA-sat, quantitative PCR on the nuclear satellite DNA; CI, 95% confidence interval (an asterisk indicates that the CI could not be estimated for cPCR specificity).