Table 1.
Optical microscopy (OM) slides examined to detect Trypanosoma cruzi infection in 841 triatomine bugs, central Brazil, 2012–2014. Fresh and Giemsa-stained slides were examined by entomological-parasitological routine surveillance (EPRS) and University of Brasília (UnB) staff in different combinations.
| OM slide combinations | Fresh slides | Stained slides | Bugs1 | ||
|---|---|---|---|---|---|
| EPRS | UnB | EPRS | UnB | ||
| Fresh EPRS + Fresh UnB | • | • | 19 | ||
| Fresh EPRS + Stained EPRS | • | • | 29 | ||
| Fresh EPRS + Stained UnB | • | • | 67 | ||
| Fresh UnB + Stained UnB | • | • | 22 | ||
| Fresh EPRS + Fresh UnB + Stained UnB | • | • | • | 334 | |
| Fresh EPRS + Stained EPRS + Stained UnB | • | • | • | 370 | |
| Total (slides/bugs) | 819 | 375 | 399 | 793 | (2386/841) |
1All tested also by one conventional and two real-time quantitative PCRs. •Slide read.