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. 2018 Jan 9;8:211. doi: 10.1038/s41598-017-18584-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fe²⁺ efflux from rat kidney cortex mitochondria is strongly reduced by an inward-directed proton gradient. PGSK-loaded mitochondria (pH 7.0_i) from rKC were suspended in pH 6.2_o, 7.0_o, or 7.6_o assay buffers and immediately assayed for quenching induced by 4 µM Fe²⁺. (A) Representative traces of the fluorescence time course are shown. Fluorescence data were normalized to the respective initial value of the second phase. (B) Statistical analyses of second phase PGSK quenching. Initial slopes of quenching, as indicated by the 20s-scale bar in (A), are plotted for pH6.2 and pH7.6, and represent means ± SE of 10–11 measurements in 3 independent experiments. ***P < 0.001. RFU, relative fluorescence units.