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. 2018 Jan 9;8:209. doi: 10.1038/s41598-017-18638-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation and characterization of the recombinant MDV mutants. (A) Overview of MDV genome with a focus on the vTR with its eight conserved regions (CR1-CR8). Recombinant viruses that either lack the entire vTR (v∆vTR), harbor EBER-1 (vEBER-1) or EBER-2 (vEBER-2) instead of vTR are shown below. The vTR sequences were completely restored in the revertant virus (vRev). (B) Plaque size assays of indicated recombinant viruses. The plaque sizes are shown as box plots with minimums and maximums. Results are shown as the means of three independent experiments (p > 0.05; one-way ANOVA, n = 150). (C) Multi-step growth kinetics of indicated viruses. The average titer and standard deviations (error bar) are shown of triplicates of one independent experiment (p > 0.05; Kruskal-Wallis test).