Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 9;8:126. doi: 10.1038/s41598-017-17786-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Scn8a knockdown reduces GFAP expression in a mouse model of MTLE. (a) Representative images of hippocampi following kainic acid (KA) and either control shRNA-scram (left column) or shRNA-Scn8a (right column) injections. DAPI staining (top) shows the hippocampal ultrastructure. GFAP immunoreactivity (bottom) was used to compare the extent of reactive gliosis in AAV-treated MTLE mice. Scale bar, 300 µm. Inset images show CA3, CA1, and DG regions of the ipsilateral (left panel) and contralateral (right panel) hippocampi following treatment with either shRNA-scram (top row) or shRNA-Scn8a (bottom row). Scale bars, 100 µm. Approximately 50% reduction in number of cells (b,c) and neurons (d,e) in the ipsilateral hippocampus were observed in MTLE animals treated with either shRNA-scram (blue) or shRNA-Scn8a (red) compared to surgery-only controls (gray). (f,g) Quantification of optical density (O.D.) of normalized GFAP immunoreactivity in the contralateral and ipsilateral hippocampi in surgery-only (gray) or MTLE animals treated with either shRNA-scram (blue) or shRNA-Scn8a (red). GFAP expression was comparable between the surgery-only controls (gray) and shRNA-Scn8a-treated MTLE mice (red) in the ipsilateral hippocampus. In contrast, GFAP expression was significantly increased in the shRNA-scram-treated MTLE mice compared to surgery-only controls in both the ipsilateral and contralateral hippocampus. Non-parametric Kruskal-Wallis test followed by Dunn’s multiple comparisons test (NeuN: N = 4–5/group, DAPI and GFAP: N = 6–8/group). All data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, N.S, not significant.