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. 2018 Jan 9;8:212. doi: 10.1038/s41598-017-18569-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Application of HybTrack to track mRNAs in neurons. (a) Image of mRNAs in a dendrite and kymograph (x-t) generated from the time-lapse image. Scale bar, 10 µm (horizontal) and 1 min (vertical). (b–d) Tracking results from u-Track (b), TrackNTrace (c) and HybTrack (d). Particle trajectories obtained from each program are overlaid on the image (upper panels) and the kymograph (lower panels). Each trajectory is shown in a different color. (e) mRNA trajectories detected by HybTrack are plotted on the time-averaged image. White dashed lines outline the dendrite. (f) The mean squared displacement of 10 mRNAs detected by HybTrack. The error bars are calculated as described in the Methods section. (g) Histograms of diffusion coefficient of diffusive mRNAs in the baseline (upper panel) and after bicuculline treatment (lower panel). (h) Effect of bicuculline treatment on the mean diffusion coefficient of diffusive mRNAs in the dendrites. Error bars represent SEM (n = 5 dendrites; P = 0.059, pairwise t-test).