TABLE 1 .
Fate of A. tropicalis ingested by D. melanogaster following administration of A. tropicalis at low densitya
Replicate expt | Fly treatment | No. of flies | Proportion of bacteria: |
||
---|---|---|---|---|---|
Egested | Retained | Lysed | |||
1 | Axenic | 50 | 0.11 | 0.09 | 0.80 |
1 | Axenic | 49 | 0.10 | 0.03 | 0.87 |
3 | Axenic | 50 | 0.26 | 0.09 | 0.65 |
Mean ± SEM | 0.18 ± 0.08 (0.12 ± 0.08) |
0.08 ± 0.02 (0.07 ± 0.01) |
0.74 ± 0.09 (0.81 ± 0.09) |
||
2 | Gnotobiotic | 45 | 0.64 | 0.10 | 0.25 |
3 | Gnotobiotic | 49 | 0.42 | 0.16 | 0.42 |
Mean ± SEM | 0.53 ± 0.11 | 0.13 ± 0.03 | 0.34 ± 0.09 |
Across three separate replicate experiments, four samples from axenic flies and two samples from gnotobiotic flies were collected and the proportions of ingested bacteria that were egested, retained, and lysed were calculated (e.g., the number of cells egested divided by the number of cells ingested; Text S1C to F). Summary statistics (mean ± SEM) were calculated across the replicate experiments performed over multiple days. The results presented for axenic flies omit one outlier replicate experiment in which no bacteria were recovered from fecal samples. Summary statistics for axenic flies with the outlier included are in parentheses.