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Titration of acute-phase tick-borne disease (TBD) samples in indirect immunofluorescence assay (IFA) to determine optimal screening dilutions. (a) Serial 2-fold dilutions of acute-phase TBD sample with Powassan virus (POWV) plaque reduction neutralization test (PRNT) titer of 1:320 to determine optimal screening dilution for IgM IFA. (b) Serial 2-fold dilutions of acute-phase TBD sample with POWV PRNT titer of 1:160 to determine optimal screening dilution for IgG IFA.
