FIG 3 .

The por1Δ mutation affects Gal83 nuclear enrichment. (A to C) Localization of Gal83-GFP was determined by fluorescence microscopy after growth to mid-log phase in selective SC containing 2% glucose (Glu) and then following a 20-min shift to an otherwise identical medium containing 3% ethanol and 2% glycerol (EG) instead of glucose. The cells were imaged by differential interference contrast (DIC) microscopy. Nuclei were stained with DAPI. Scale bars, 5 μm. (D) Cells were grown as described for panels A to C, and Gal83-GFP protein levels as well as Thr210 phosphorylation (PT210) and total levels of Snf1 were analyzed by immunoblotting. WT, wild type.