FIG 5 .

Overexpression of Por2 suppresses the por1Δ mutation for the Snf1 nuclear enrichment defect. (A to D) Cells expressing Snf1-GFP and either overexpressing Por2-V5 or carrying the corresponding empty vector were grown to mid-log phase in selective SC medium containing 2% glucose (Glu) and then shifted to an otherwise identical medium containing 3% ethanol and 2% glycerol (EG) instead of glucose. The cells were imaged by differential interference contrast (DIC) microscopy. Nuclei were stained with DAPI. Scale bars, 5 μm. (E) Cells expressing Snf1-GFP and overexpressing Por2-V5 (+) or carrying the corresponding vector (−) were shifted to ethanol-glycerol as described above, and the levels of Snf1-GFP phosphorylated at Thr210 (PT210), total Snf1-GFP, and Por2-V5 were analyzed by immunoblotting. WT, wild type.