FIG 8 .

Requirement of Gal83 for suppression of the por1Δ mutation. (A) Hyperactive LexA-Snf1-G53R ameliorates the effects of the por1Δ mutation in a Gal83-dependent manner. Transformants were grown in selective SC containing 2% glucose to mid-log phase and then washed, and serial dilutions were spotted onto selective SC plates with either 2% glucose or a mixture of 3% ethanol and 2% glycerol as carbon sources. The plates were incubated at 30°C for 2 and 4 days, respectively. (B) Overexpression of Por2 ameliorates the effects of the por1Δ mutation, independent of Gal83. Transformants were grown as described for panel A. WT, wild type.