FIG 1 .

Recombinant ZIKV E protein expressed in Drosophila S2 cells after purification using 4G2 MAb. (A) Coomassie blue-stained SDS PAGE gel (4 to 12% polyacrylamide) featuring ZIKV E protein. The molecular weights of the standard marker sizes are shown in kilodaltons (kDa). Purified ZIKV E of approximately 1 μg migrated as a single band. (B) Western blot of purified ZIKV E protein loaded at the same concentration as in panel A. Replicate Western membranes were probed with 4G2 MAb, convalescent ZIKV-infected mouse, or NHP sera.