FIG 3 .

Antibody responses to recombinant ZIKV E protein in three strains of mice. (A) Vaccination schedule. (B) Experimental groups. Alum, 2% Alhydrogel adjuvant. (C) ZIKV E-specific IgG antibodies in the sera from three strains of mice were measured after each immunization using MIA and are plotted as GMTs with 95% CI of total IgG titers. S.D., standard deviations. (D) The neutralizing titers in mouse sera at 2 weeks after the second immunization were measured by PRNT and plotted as the PRNT₅₀, the calculated dilution of serum that gave 50% inhibition. Statistical analysis was conducted using unpaired t tests and Prism (GraphPad) software. PRNT₅₀ values were generated using a fixed, or variable-slope, sigmoidal dose-response model. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.